Evaluation of HIV-1 polymerase chain reaction (PCR) technique using dried blood spots (DBS) for diagnosis of perinatal transmission of HIV
نویسندگان
چکیده
Background Effective health care delivery to the majority of perinatally exposed infants is hampered by lack of access to accurate HIV diagnosis in infancy. Polymerase chain reaction is the most sensitive test to diagnose HIV-1 infection in children born to HIV seropositive mothers. The purpose of this study was to assess the feasibility and accuracy of using dried blood spot (DBS) technology in performing HIV-1 DNA PCR using Roche Amplicor HIV-1 DNA PCR version 1.5 for diagnosis in children less than 18 months of age.
منابع مشابه
Evaluation of a single round polymerase chain reaction assay using dried blood spots for diagnosis of HIV-1 infection in infants in an African setting
BACKGROUND The aim of this study was to develop an economical 'in-house' single round polymerase chain reaction (PCR) assay using filter paper-dried blood spots (FP-DBS) for early infant HIV-1 diagnosis and to evaluate its performance in an African setting. METHODS An 'in-house' single round PCR assay that targets conserved regions in the HIV-1 polymerase (pol) gene was validated for use with...
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BACKGROUND There is a paucity of data on the prevalence of hepatitis C virus (HCV) in children, particularly in sub-Saharan Africa. A major obstacle in resource-limited settings for polymerase chain reaction (PCR) testing is the necessity for specimen transportation and storage at low temperatures. There are numerous recent studies of using real-time HCV PCR for diagnosis and screening of plasm...
متن کاملComparative evaluation of Amplicor HIV-1 DNA test, version 1.5, by manual and automated DNA extraction methods using venous blood and dried blood spots for HIV-1 DNA PCR testing.
Human immunodeficiency virus (HIV) DNA polymerase chain reaction (PCR) test using venous blood sample has been used for many years in low resource settings for early infant diagnosis of HIV infection in children less than 18 months. The aim of this study was to evaluate and compare the performance characteristics of Amplicor HIV-1 DNA assay version 1.5 following processing of venous blood and d...
متن کاملDried blood spots improve access to HIV diagnosis and care for infants in low-resource settings.
Effective health care delivery to the majority of perinatally exposed infants worldwide, including those enrolled in prevention of mother-to-child transmission programs, is hampered by lack of access to an HIV diagnosis in infancy. Dried blood spot collection from young infants for centralized HIV polymerase chain reaction (PCR) testing is attainable in low-resource settings, provided PCR metho...
متن کاملA study to implement early diagnosis of HIV infection in infants born to infected mothers.
A protocol for detecting HIV DNA from specimens collected on filter papers and the effect of storage temperatures on determination of HIV DNA from dried blood spots has been developed and optimized. Blood specimens collected from HIV-1 infected and normal persons were spotted onto blood collection cards (Whatman BFC 180). The HIV DNA was extracted by phenol-chloroform-isoamyl alcohol and was de...
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